Dr. Sebanti Gupta
PhD: School of Biological Sciences, Nanyang Technological University, Singapore
MSc: Department of Biophysics, Raja Bazar Science College, University of Calcutta, India
Dr. Gupta received her Ph.D. from Nanyang Technological University, in 2013. She worked under the direction of Dr. Surajit Bhattacharjya in the high field magnetic laboratory to understand the protein folding and dynamics and their functional implications. Her postdoctoral work in the same lab revealed the serine phosphorylation dependent regulation of integrin function. Later she joined the group of Dr. LiuChuan Fa, where she was exposed to solid-phase peptide synthesis and protein/peptide ligation techniques. Her final postdoctoral training was with Dr. Robert Tycko at the prestigious Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, USA. She established intein based protein ligation method for HIV-1 mature and immature capsid protein there. These specially labeled samples were used for solid-state NMR based characterization of the immature and mature capsid core and to determine the mechanism of action of the maturation inhibitors of HIV-1. Dr. Gupta holds certificates on ‘Research Ethics’ from NIH which is personalized for scientists.
In her present assignment at YRC, she is setting up a Structural Biophysics Laboratory to work on the bacterial and viral proteins and protein modifications aided towards the biomedical needs.
- Assistant Professor (Nov 2019- till date) – Yenepoya Research Center, Yenepoya (Deemed to be University), Mangalore, India
- Visiting Fellow, Postdoctoral (Aug 2015 – Oct 2018) – National Institutes of Health, Bethesda, MD, USA
- Research Fellow, Postdoctoral (March 2013 – March 2015) – School of Biological Sciences, Nanyang Technological University, 60 Nanyang Ave, Singapore
- Research Assistant (Nov 2007 – Aug 2008) – Department of Biophysics, Raja Bazar Science College, University of Calcutta, India
- Visiting Fellowship, National Institutes of Health, USA (May 2015 – Oct 2018))
- NIH Travel Grant for poster presentation atthe 4thU.S.-Canada Winter School on Biomolecular Solid-State NMR, Stowe, VT, USA (Jan 2016)
- Research Fellowship, Nanyang Technological University, Singapore (March 2013 – March 2015)
- Student Bursary from ‘Institute of Pasteur’ for poster presentation at the XXVth International Conference on Magnetic Resonance in Biological System, Lyon, France. (Aug 2012)
- NTU Travel Grant for poster presentation at the XXVth International Conference on Magnetic Resonance in Biological System, Lyon, France. (Aug 2012)
- Nanyang Technological University Research Scholarship, Singapore (Jan 2009 – Dec 2012, PhD program)
- Qualified in Graduate Record Examination (GRE) with the total score of [verbal 560 out of 800 and Quant’s 790 out of 800] conducted by Educational Testing Service (ETS) (year 2008)
- Merit Medal for academic excellence, Lady Brabourne College, University of Calcutta, India (Bachelor’s Program 2005)
Research Interest/ Area
Structural Virology, Protein/peptide chemistry, protein folding and dynamics, Solid state Nuclear Magnetic Resonance Spectroscopy, Solution state Nuclear Magnetic Resonance Spectroscopy, Drug designing, recombinant proteins, Structural modelling, Biophysics and Bioinformatics
- Structural Virology and peptide therapeutics
The objective of our lab is to study the biomolecules. We are especially interested in dissecting the role of viral proteins in viral replication cycle and their interaction with host factors to decipher the new therapeutic targets using multidimensional solution-state and solid-state NMR spectroscopy, Electron Microscopy, Dynamic Light Scattering, Circular Dichroism, Isothermal Calorimetric Technique, Fluorescence spectroscopy and other biophysical tools. The following areas will be explored towards achieving this goal;
- Biophysical Chemistry: Protein/peptide structure and dynamics
- Structural Virology:Structural characterization of the viral proteins
- Drug designing: Biophysical cauterization of the cellular and viral determinant for drug designing
- Biomaterials: Protein ligation and modifications for biomedical application
- Gupta S, Louis JM., Tycko R. Effects of an HIV-1 Maturation Inhibitor on the Structure and Dynamics of CA-SP1 Junction Helices in Virus-Like Particles. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (19), 10286-10293.
- Gupta S, Tycko R. Segmental Isotopic Labeling of HIV-1 Capsid protein Assemblies for Solid State NMR. Journal of Biomolecular NMR, (2018) 70:103–114.
- Gupta, S.; Chit, J. C.-Y.; Feng, C.; Bhunia, A.; Tan, S.-M.; Bhattacharjya, S. An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding. Scientific Reports 2015, 5, 11630.
- Gupta S, Bhattacharjya S. Characterization of the near native conformational states of the SAM domain of Ste11 protein by NMR spectroscopy. Proteins: Structure, Function and Bioinformatics2014, 82(11):2957-2969.
- Gupta S, Bhattacharjya S. NMR characterization of the near native and unfolded states of the PTB domain of Dok1: alternate conformations and residual clusters, PLoS One.2014,9(2): e90557.