Dr. Shobha D.
Plant molecular biology
Dr. Shobha D., obtained her Ph.D. from Directorate of Cashew Research (ICAR), Puttur and Dept. of Applied Botany, Mangalore University. As part of her thesis work, she worked mainly on DNA based molecular markers especially RAPD, ISSR and microsatellite markers. She employed these markers in genetic mapping, development of molecular markers for improvement of yield in cashew. She has been working on plant molecular biology for 10 years and successfully switched her research career from plant molecular biology to biomedical field. During her Post-Doctoral experience, she worked on biogenesis and structural characterization of surface macromolecules from Helicobacter pylori for translational medical applications. At YRC, she is involved in the identification of candidate biomarkers and novel therapeutic targets in Renal Cell Carcinoma (RCC) using circulating tumour cells, Acute Kidney injury (AKI) and different kidney stone diseases using quantitative proteomic and metabolomic analysis in collaboration with the clinicians at Yenepoya Medical College.
Dr. Shobha D. worked as Post Doctoral Fellow in the Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. She mastered advanced molecular biology techniques and carried out analysis of Type IV secretion systems in Helicobacter pylori, which causes gastric cancer in humans. She generated the mutants for type IV secretion systems, performed cell culture and studied the gene expression for disease based signaling pathways using RT-PCR, which resulted in international journal publication.
Awards / Honors
- Post Doctoral Fellow, Center for Systems Biology and Molecular Medicine, Mangalore, India, 2017
- Post Doctoral Fellowship, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, 2014-2016
- Senior Research Fellow, CPCRI (ICAR), Regional Station, Vittal, 2011-2014
- Junior Research Fellow, DCR (ICAR), Puttur, 2006-2011
Proteomics and metabolomics in infectious diseases, cancer biology, signaling pathways, therapeutic targets in different kidney diseases
Currently involved in the development of biomarkers and identification of novel therapeutic targets in different kidney diseases including acute kidney Injury, different kidney stones and Renal Cell Carcinoma (RCC) using quantitative proteomic and metabolomic approaches. She is involved in the establishment of isolation and ex vivo culture of circulating tumour Cells (CTCs) from peripheral blood of cancer patients, which can be used as a diagnostic and prognostic marker for various cancer such as prostate cancer, bladder cancer, and renal cell carcinoma. Ultimately, it may help to develop easily accessible tools as point of care testing device to provide comprehensive information regarding different stages of cancer using liquid biopsies instead of using conventional biopsies. She is also involved in the development of molecular based diagnostic method for a neglected disease ‘Scrub typhus’ using conventional PCR. She is also involved in curation of molecular reactions associated with some important receptors and its ligands from publicly available literature to construct pathway map. It will help to wider scientific community to readily access data and contribute further to this pathway. Apart from this she is also involved human brain and eye proteome project. The proteogenomic analysis also allows us to refine genome annotation, correction of existing protein-coding region and identification of novel genes.
Publications Full List :
- Sahu, A., Gopalakrishnan, L., Gaur, N., Chatterjee, O., Mol, P., Modi, P. K., Dagamajalu, S., Advani, J., Jain, S. and Prasad, T. S. K. (2018). The 5-Hydroxytryptamine signaling map: an overview of the serotonin-serotonin receptor-mediated signaling network. Journal of Cell Communication and Signaling.
- Thimmappaiah, Dagamajalu, S., Mohana G.S, Adiga, J.D. & Bhat, P.G. (2016).Fingerprinting of released varieties of cashew based on DNA markers. Vegetos.
- Dieye, Y., Mbengue, B., Dagamajalu, S., Fall, M. M., Loke, M. F., Nguer, C. M., Thiam A., Vadivelu, J. & Dieye, A. (2016). Cytokine response during non-cerebral and cerebral malaria: evidence of a failure to control inflammation as a cause of death in African adults. Peer J.
- Dagamajalu, S., Thimmappaiah & Jose, C.T. (2013). Molecular characterization and association analysis in cashew using RAPD and ISSR markers. Journal of Plantation Crops.
- Dagamajalu, S. & Thimmappaiah. (2011). Identification of RAPD markers linked to nut weight and plant stature in cashew. Scientia Horticulturae.129, 637-641.
- Thimmappaiah, Dagamajalu, S., Sequiera, M.G. & Goveas, S.W. (2010). Cashew (Chapter 15). In: Advances in Hortuculture Biotechnology— Molecular Markers and Marker Assisted Selection— Fruit Crops, Plantation Crops and Spices (Volume III), pp 330, Westville Publishing House, New Delhi.
Number of prejects: 12