Dr. Saketh Kapoor
Regenerative Stem cells
MSc (Area): Biotechnology
Mr. Saketh Kapoor joined Yenepoya Research Centre as a Ph.D. scholar and he is working on proteomic profiling of CD34+ regenerative stem cells isolated from all the three germ layers (ectoderm, endoderm and mesoderm) and their therapeutic application. He is a recipient of the prestigious EMBO Short-Term Fellowship which allowed him to visit the lab of Prof. Antonio Mazzocca, University of Bari, Italy. He has been instrumental in developing targeted proteomics assays at Yenepoya Research Centre for the detection of low abundant pathogen derived peptides in complex biological samples using high-resolution mass spectrometry. His expertise is in molecular biology techniques, sample preparation for proteomic analysis, animal cell culture techniques, mass-spectrometry derived data analysis and user-end bioinformatics.
Prior to joining this centre, he worked as a Junior Research Fellow in Indian Institute of Science (IISc), Bangalore (2011-2015) where he worked on genetic analysis of various human disorders such as Parkinson’s disease, Coats plus syndrome, Neuroaxonal Dystrophy, Congenital Hereditary Endothelial Dystrophy, Wilson’s disease, Waardenburg syndrome and Duchenne Muscular Dystrophy. He obtained his M.Sc. degree in Biotechnology (2008-2010) from Bangalore University, Bangalore, India.
- EMBO Short-Term Fellowship for three months to attend University of Bari, Italy (2018)
- Third rank in quiz competition held during the Targeted Proteomics International Symposium on February 25Th, 2018 at Indian Institute of Technology (IIT) Bombay, India
- “Best Poster Award” at KSHEMA STEMCON-2017 organized by NITTE University, Mangalore (2017)
- First rank in the event “Budding Scientist- Best Hypothesis Award” organized by Yenepoya Research Centre, Yenepoya University on the occasion of National Science day (2016)
Stem cells, regenerative medicine, mass spectrometry, genetic analysis, molecular biology, scientific writing
Bone marrow has remained an exclusive source for CD34+ stem/progenitor cells used in treating various haematological disorders. Our team at Stem cell and Regenerative Medicine Centre, Yenepoya (Deemed to be University) has successfully established the isolation of CD34+ cells from various tissues. My research focus is to carry out an in depth molecular characterization of CD34+ stem/progenitor cells isolated from mouse tissue samples belonging to three germ layers. CD34+ cells will be isolated from each of the tissue samples using fluorescence activated cell sorting method. To understand the proteome and/or transcriptome of these cells we will use techniques such as high-resolution mass spectrometry and Next-Gen Sequencing platforms. Regenerative potential of the isolated cells will be validated by transplanting these cells into Mdx mice and their roles in ameliorating dystrophic phenotype will be studied. Further, we also plan to utilize the gene editing technique CRISPR/Cas9 to characterize genes that display distinct expression patterns in CD34+ cells isolated from three germ layers.
- Kapoor S, Subba P, Shenoy PS, Bose B (2020). Sca1+ cells as direct isolate (ex vivo) versus in vitro cultured exhibit differential proteomic signatures in murine skeletal muscle. bioRxiv; doi: https://doi.org/10.1101/2020.12.02.409110
- Kapoor S, Shenoy PS and Bose B (2020). Presence of mesenchymal and hematopoietic stem cell markers in murine liver and muscle: an ex vivo and in vitro study. Journal of Natural Remedies; 21(2):91-100.
- Kapoor S* and Subba (2020). Predicted peptide patterns from the SARS-CoV-2 proteome for MS-MS based diagnosis. Bioinformation; 16(6), 477-482(*Co-corresponding author).
- Singh, N., Kallollimath, P., Shah, M., Kapoor, S., Bhat, V., Viswanathan, L., Nagappa, M., Bindu, P.S., Taly, A.B., Sinha, S., Kumar, A. (2019). Genetic analysis of ATP7B in 102 south Indian families with Wilson disease. (Accepted in PLoS One).
- Yadav, R., Kapoor, S., Madhukar, M., Manja, R., Pal, P.K., Kumar, A. Genetic analysis of the Glucocerebrosidase gene in Indian patients with Parkinson’s disease. (2018). Neurology India. 66(6): 1649-1654.
- Shenoy, P.S., Sen, U.#, Kapoor, S.#, Ranade, A.V., Chowdhury, C.R., Bose, B. (2018). Sodium fluoride induced skeletal muscle changes: Degradation of proteins and signaling mechanism. Environmental Pollution. 244: 534-548. (#Equal contribution).
- Pinto, S.M., Verma, R., Advani, J., Chatterjee, O., Patil, A.H., Kapoor, S., Subbannayya, Y., Raja, R., Gandotra, S., Prasad, T.S.K. (2018). Integrated multi-omic analysis of M. tuberculosis H37Ra redefines virulence attributes. Frontiers in Microbiology. 9: 1314.
- Subbannayya, Y., Karthikkeyan, G., Pinto, S. M., Kapoor, S., Tyagi, A., Pervaje, S. K., Pervaje, R. and Prasad, T. S. K. (2018). Global metabolite profiling and network pharmacology of Triphala identifies neuromodulatory receptor proteins as potential targets. Journal of Proteins and Proteomics. 9(2): 101-114.
- Kapoor, S., Shah, M.H., Singh, N., Rather, M.I., Bhat, V., Gopinath, S., Bindu, P.S., Taly, A.B., Sinha, S., Nagappa, M., Bharath, R.D., Mahadevan, A,, Narayanappa, G., Chickabasaviah, Y.T., Kumar, A. (2016). Genetic Analysis of PLA2G6 in 22 Indian Families with Infantile Neuroaxonal Dystrophy, Atypical Late-Onset Neuroaxonal Dystrophy and Dystonia Parkinsonism Complex. PLoS One. 19;11(5): e0155605.
- Netravathi, M., Kumari, R., Kapoor, S., Dakle, P., Dwivedi, M.K., Roy, S.D., Pandey, P., Saini, J., Ramakrishna, A., Navalli, D., Satishchandra, P., Pal, P.K., Kumar, A., Faruq, M. (2015). Whole exome sequencing in an Indian family links Coats plus syndrome and dextrocardia with a homozygous novel CTC1 and a rare HES7 variation. BMC Medical Genetics. 10; 16: 5.
- Kodaganur, S.G., Kapoor, S., Veerappa, A.M., Tontanahal, S.J., Sarda, A., Yathish, S., Prakash, D.R., Kumar, A. (2013). Mutation analysis of the SLC4A11 gene in Indian families with congenital hereditary endothelial dystrophy 2 and a review of the literature. Molecular Vision. 2; 19:1694-706.
- Kapoor, S., Bindu, P.S., Taly, A.B., Sinha, S., Gayathri, N., Rani, S.V., Chandak G.R., Kumar, A. (2012). Genetic analysis of an Indian family with members affected with Waardenburg syndrome and Duchenne muscular dystrophy. Molecular Vision. 18: 2022-2032.