The following figure depicts the mechanisms and subcellular locations of various functional assays commonly used for the charecterization of in vitro oxygen-glucose deprivation model of brain cells. These assays are:(1) DCFDA assay, (2) MTT assay, (3) MPT pore opening assay, (4) LDH assay, (5) FITC Annexin V - PI staining, (6) Trypan blue dye exclusion, (7) Tunnel assay. MTT assay and LDH assay are the two most frequently used techniques.

Reference: Babu M, Singh N, Datta A. (2022). Molecular Neurobiology

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